Locations away from Platanthera chlorantha (PS1 and PS2, PB1–PB4, circles) and you can Cephalanthera rubra (CK1 and you will CK2, CB1–CB7, triangles) populations for the northern-east Poland.

Data urban area and you can sampling

I investigated six P. chlorantha and nine C. rubra communities into the northern-eastern Poland (Bialowieza and you will Knyszynska Primeval Tree, Szeszupa lake area) inside the pure, semi-absolute and you will anthropogenic communities out of federal and you may surroundings parks, supplies and you will safe components, such as for example Natura 2000 sites ( Fig. 1). Despite the reality he is located in secure section, of numerous can be found towards train embankments, together routes and you may routes into the woods or perhaps in clearings.

The latest testing processes depended on populace proportions. Leaf trials off almost all ramets within this populations each and every types was in fact taken (but people PS2; Dining table step 1); no examples were taken from damaged or extremely more youthful some body. A hundred and ninety-seven samples regarding P. chlorantha and you can 95 products away from C. rubra were accumulated. Leaf cells try continued ice until it can be held from the ?80 °C, pending allozyme investigation. All-collected trials were used having allozyme studies.